DRD4 Antikörper (AA 215-228)

Produktnummer ABIN7043110

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch DRD4 in WB. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für DRD4 Antikörper (AA 215-228) (ABIN7043110)

Target: DRD4 (Dopamine Receptor D4 (DRD4))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DRD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-DRD4 Antikörper

Bindungsspezifität: AA 215-228

Verwendungszweck: A Rabbit Polyclonal Antibody to D4 Dopamine Receptor

Spezifität: 3rd intracellular loop

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 9,14 amino acid residues identical

Produktmerkmale: Anti-D4 Dopamine Receptor Antibody is directed against an epitope located at the 3rd intracellular loop of the rat D4 dopamine receptor. Anti-D4 Dopamine Receptor Antibody (ABIN7043110 and ABIN7044233) can be used in western blot analysis, and recognizes DRD4 from rat and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RRWEAARHTKLHSR, corresponding to amino acid residues 215-228 of rat DRD4 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7235246)

Blocking Peptide: (ABIN7235246)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu DRD4

Target: DRD4 (Dopamine Receptor D4 (DRD4))

Andere Bezeichnung: DRD4

Hintergrund: DRD4, D4DR, D(2C) dopamine receptor,The dopamine neurotransmitter belongs to the catecholamines and can therefore be further converted into adrenaline and noreadrenaline. Dopamine has various physiological roles, including learning and memory, motor output and endocrine regulation. It does so by binding and activating dopamine receptors which belong to the G-protein coupled receptor superfamily (GPCR)1.The D4 dopamine receptor (DRD4) belongs to the D2-like family as do D2 and D3 dopamine receptors. Like all GPCRs, it has seven transmembrane spanning membrane regions. Structure-wise, members of the family share high homology in the transmembrane domains and lower homology in the extracellular N-terminal and the intracellular C-terminal domains. Notably, the coding region of the 3rd intracellular loop of DRD4 is known to undergo extensive polymorphism2.Like many GPCRs, each dopamine receptor subtype can react with more than one G-protein giving rise to different signaling possibilities3. Whereas D2-like dopamine receptors are generally considered to couple to Gi, and therefore inhibit adenylyl cyclase, the signaling through DRD4 is complicated due to the polymorphisms in the 3rd intracellular loop. It seems that this region is important to G-coupling as different polyphormisms in the region influence the ability of DRD4 to couple to adenylyl cyclase and G-proteins4,5. D4 dopamine receptors also influence Ca2+ levels3,6. They could also interact with G-protein couple inwardly rectifying K+ channel to ultimately cause a decrease in the firing rate of neurons7.The distribution of DRD4 mostly includes the brain and is mainly found post-synaptically in dendritic shafts and spines of mammalian striatum8.

Alternative names: D4 Dopamine Receptor, DRD4, D4DR, D(2C) dopamine receptor

Gen-ID: 25432

NCBI Accession: NM_000797

UniProt: P30729

Pathways: cAMP Metabolic Process, Synaptic Membrane, Proton Transport, Photoperiodism, Negative Regulation of Transporter Activity