Cortactin Antikörper (Intracellular)

Produktnummer ABIN7887044

Der Kaninchen Polyklonal anti-Cortactin Antikörper (ABIN7887044) detektiert spezifisch Cortactin in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für Cortactin Antikörper (Intracellular) (ABIN7887044)

Target: Cortactin (CTTN)

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cortactin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Cortactin Antikörper

Bindungsspezifität: AA 346-359, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Cortactin

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,human - identical

Produktmerkmale: Anti-Cortactin Antibody (ABIN7043085, ABIN7045162 and ABIN7045163) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Cortactin from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KTSNIRANFENLAK, corresponding to amino acid residues 346 - 359 of mouse Cortactin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200-1:1000

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235169)

Blocking Peptide: (ABIN7235169)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Cortactin

Target: Cortactin (CTTN)

Andere Bezeichnung: CTTN

Hintergrund:

Synonyms: Src substrate cortactin, Oncogene EMS1, EMS1, Amplaxin, CTTN

Description: Cortical acting binding protein, Cortactin, is an actin-binding protein involved in cytoskeletal regulation1. Cortactin is a 63-65 kDa protein that consists of four major domains: N-terminal acidic (NTA), 6.5 tandems repeat, proline-rich domain, and the C-terminal Src homology 3 domains (SH3 domains)2 and it is regulated by post translational modifications such as phosphorylation and acetylation1.The actin filament network provides mechanical support as well as protrusive and contractile forces essential for directed cell motility and cell and tissue morphogenesis. Cortactin activates the Arp2/3 complex, the main regulator of actin polymerization, and thereby regulates the assembly of branched actin3.Overexpression of cortactin was shown to enhance cell migration, invasion, and tumor cell metastasis. It is also overexpressed in various types of cancer such as breast cancers as well as head and neck tumors1.

Gen-ID: 13043

NCBI Accession: NM_138565

UniProt: Q60598

Pathways: MAPK Signalweg