CNTF Antikörper (AA 164-177)

Produktnummer ABIN7043070

Dieses Anti-CNTF-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CNTF in WB, IHC, IF und IC. Geeignet für Human.

Kurzübersicht für CNTF Antikörper (AA 164-177) (ABIN7043070)

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CNTF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Produktdetails anti-CNTF Antikörper

Bindungsspezifität: AA 164-177

Verwendungszweck: A Rabbit Polyclonal Antibody to CNTF

Spezifität: C-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,mouse - identical

Produktmerkmale: Anti-CNTF Antibody is directed against an epitope of human CNTF. Anti-CNTF Antibody (ABIN7043070 and ABIN7044789) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize CNTF from human, rat and mouse samples. Anti-CNTF Antibody specifically recognizes CNTF and not related cytokines.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ELSQWTVRSIHDLR, corresponding to amino acid residues 164-177 of human CNTF

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7235138)

Blocking Peptide: (ABIN7235138)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu CNTF

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Andere Bezeichnung: CNTF

Hintergrund: Ciliary neurotrophic factor,CNTF is a polypeptide trophic factor, member of the alpha-helical cytokine superfamily1. It was initially purified from the chick eye on the basis of its ability to promote survival of E8 chick ciliary ganglion neurons in culture2. CNTF is synthesized by glia both in the CNS and PNS3 and it has been demonstrated that CNTF is ubiquitously distributed in neurons and glia throughout the rodent brain4. CNTF effects are mediated by a tripartite receptor complex consisting of two signal-transducing subunits (leukemia inhibitory factor receptor, gp130) and a CNTF-specific ligand-binding-subunit (CNTFR)5.CNTF can support the survival of many different cell populations within the PNS and the CNS6. in vitro, CNTF promotes proliferation and neuronal specifications in hippocampal neurons. in vivo, it supports the viability of non-primate motor neurons7, induces sprouting of cholinergic motor neurons8 and delays neural degeneration in genetic models of motor neuron disease9. In addition, it is involved in the development stage of astrocytes and oligodendocytes10.

Alternative names: CNTF, Ciliary neurotrophic factor

Gen-ID: 1270

NCBI Accession: NM_000614

UniProt: P26441

Pathways: JAK-STAT Signalweg