CLIC4 Antikörper (Intracellular, N-Term)

Produktnummer ABIN7886227

Der Kaninchen Polyklonal anti-CLIC4 Antikörper wird verwendet zum Nachweis von CLIC4 in Proben von Human. Er wurde validiert für WB, IF und IC.

Kurzübersicht für CLIC4 Antikörper (Intracellular, N-Term) (ABIN7886227)

Target: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CLIC4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Produktdetails anti-CLIC4 Antikörper

Bindungsspezifität: AA 8-20, Intracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to CLIC4 Channel

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,mouse - identical

Produktmerkmale: Anti-CLIC4 Antibody (ABIN7043057 and ABIN7044140) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize CLIC4 from mouse, rat, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)NGLKEEDKEPLIE, corresponding to amino acid residues 8-20 of human CLIC4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:200

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235106)

Blocking Peptide: (ABIN7235106)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu CLIC4

Target: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Andere Bezeichnung: CLIC4

Hintergrund:

Synonyms: Cl- intracellular channel 4, Intracellular chloride ion channel p64H1

Description: Chloride intracellular channel (CLIC) is a family of proteins encoded by six different genes (CLIC1-6)1-3.The localization of the various CLIC channels remains elusive and controversial since they can be either found in a soluble form in the cytoplasm or as integral membrane proteins (in intracellular compartments such as the nuclear membrane or the endoplasmic reticulum for example, or associated with the plasma membrane) via their single transmembrane domain1.When membrane associated, CLIC channels indeed function as ion channels (enabling the passage of Cl- ions). Non-channel functions are also attributed to CLICs and include cell cycle regulation, cell differentiation, and apoptosis. These different roles depend on whether CLICs are membrane bound or cytosolic1. For instance, CLIC4 is involved in keratinocyte differentiation in a TGFb-dependent manner4,5, in such a way that TGFb stimulation induces the expression and nuclear localization of CLIC4 and its subsequent interaction with transcription factors4. CLIC4 is also required for growth arrest and apoptosis under stress6.CLIC4 distribution is rather ubiquitous but shows higher expression level in the skin, kidney, brain and testes7.

Gen-ID: 25932

NCBI Accession: NM_013943

UniProt: Q9Y696