MERTK Antikörper (Extracellular)

Produktnummer ABIN7042974

Produktdetails anti-MERTK Antikörper

Target: MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))

Bindungsspezifität: AA 402-416, Extracellular

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MERTK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc))

Verwendungszweck: A Rabbit Polyclonal Antibody to MERTK Receptor

Spezifität: Extracellular, N-terminus.

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat - identical, human - 13,15 amino acid residues identical

Produktmerkmale: Anti-MERTK (extracellular) Antibody (ABIN7042974, ABIN7045326 and ABIN7045327)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry. It has been designed to recognize MERTK from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KPPIKRQDGELVGYR, corresponding to amino acid residues 402 - 416 of mouse MERTK

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: BLP-TR033

Blocking Peptide: BLP-TR033

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu MERTK

Target: MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))

Andere Bezeichnung: MERTK (MERTK Produkte)

Hintergrund: Receptor tyrosine kinase MerTK, C-Mer Proto-Oncogene Tyrosine Kinase, Mer, Proto-Oncogene C-Mer,Myeloid-epithelial-reproductive tyrosine kinase, MERTK, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and MERTK) family, expressed in numerous cancers1.The extracellular N-terminal domain contains two immunoglobulin-like and two fibronectin 3 domains, followed by a hydrophobic single pass transmembrane domain, and a cytoplasmic C-terminal tail containing a tyrosine kinase domain1,2.Auto-phosphorylation induced by ligand binding influences a range of downstream signaling pathways and cellular functions2. The physiological roles of MERTK include regulation of tissue homeostasis and repair, innate immune control, and platelet aggregation. However, aberrant expression of this protein promotes neoplasia, cell cycle progression, proliferation and tumor growth, resistance to apoptosis, and promotion of tumor metastases.MERTK is involved in the activation of several canonic oncogenic signaling pathways in various types of cancers including: leukemia, non-small cell lung cancer, glioblastoma, melanoma, prostate cancer, breast cancer, colon cancer and gastric cancer1,3,4.Due to its pivotal role in cancer development and metastasis, multiple therapeutic approaches are currently in development to inhibit MERTK, including ligand "traps" and small-molecule tyrosine kinase inhibitors4.

Alternative names: Receptor tyrosine kinase MerTK, C-Mer Proto-Oncogene Tyrosine Kinase, Mer, Proto-Oncogene C-Mer

Gen-ID: 17289

NCBI Accession: NM_006343

UniProt: Q60805

Pathways: RTK Signalweg