AVPR2 Antikörper (C-Term, Intracellular)

Produktnummer ABIN7887190

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch AVPR2 in WB. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für AVPR2 Antikörper (C-Term, Intracellular) (ABIN7887190)

Target: AVPR2 (Arginine Vasopressin Receptor 2 (AVPR2))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AVPR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-AVPR2 Antikörper

Bindungsspezifität: AA 345-358, C-Term, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Vasopressin V2 Receptor

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 9,14 amino acid residues identical

Produktmerkmale: Anti-Vasopressin V2 Receptor (AVPR2) Antibody is directed against an epitope of rat Arginine vasopressin receptor 2. Anti-Vasopressin V2 Receptor (AVPR2) Antibody (ABIN7042948, ABIN7045339 and ABIN7045340) can be used in western blot analysis. It has been designed to recognize V2R from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: RHTTHSLGPQDESC, corresponding to amino acid residues 345-358 of rat AVPR2 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Cited Application: IP

Negative Control: (ABIN7234794)

Blocking Peptide: (ABIN7234794)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu AVPR2

Target: AVPR2 (Arginine Vasopressin Receptor 2 (AVPR2))

Andere Bezeichnung: AVPR2

Hintergrund:

Synonyms: V2R, AVPR V2, Antidiuretic hormone receptor, ADHR, Renal-type arginine vasopressin receptor, DIR3, Nephrogenic diabetes insipidus

Description: Vasopressin V2 receptor (V2R), or arginine vasopressin receptor 2 (AVPR2), is a G-protein-coupled receptor (GPCR), coupled to the stimulatory heterotrimeric GTP-binding protein, GS, that elevates intracellular cAMP through activation of adenylate cyclase1-3. Like all members, it has seven transmembrane domains, an extracellular N-terminus and intracellular C-terminal tail1.AVPR2 is expressed primarily in renal collecting ducts and loop of Henle of the kidney, but also in vascular smooth muscle, where it induces vasodilation. Its ligand is the anti-diuretic hormone (ADH), aka vasopressin, a hormone that originates from the posterior pituitary gland that has key roles in water homeostasis and in hypotension5.Vasopressin triggers a variety of receptors - V1R, V2R, V3R, oxytocin and purinergic receptors, which regulate the physiological water-balance. High levels of inflammatory factors in the blood, such as the case in sepsis, are known to down-regulate the vasopressin receptors3.Mutations in V2R that prevent the receptor from reaching and anchoring to the cell surface are responsible are detected in diabetes insipidus and heart failure4,6,7 just to name a few. Interestingly, it was found that female rats possess more V2R mRNA than male rats leading to increased urine osmolality and decreased urine volume in females compared with males - a finding which explains that clinical data according to which females are more prone to suffer from hyponatremia4. A recent study also showed that vasopressin interacts with V2R in sperm-cell membranes to enhance ion transport, over expression of the hormone reduced sperm motility, hence V2R also has an important part in male fertility8.

Gen-ID: 25108

NCBI Accession: NM_000054

UniProt: Q00788

Pathways: cAMP Metabolic Process