ASIC3 Antikörper (Intracellular, N-Term)

Produktnummer ABIN7887112

Der Kaninchen Polyklonal anti-ASIC3 Antikörper (ABIN7887112) detektiert spezifisch ASIC3 in WB, IHC, IF und IC. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für ASIC3 Antikörper (Intracellular, N-Term) (ABIN7887112)

Target: ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ASIC3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Güteklasse: KO Validated

Produktdetails anti-ASIC3 Antikörper

Bindungsspezifität: AA 2-16, Intracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to ASIC3 Channel

Kreuzreaktivität: Human, Maus, Ratte

Homologie: 15 amino acid residues identical, human - 11,Mouse - 14

Produktmerkmale: Anti-ASIC3 Antibody (ABIN7042917, ABIN7045243 and ABIN7045244) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize ASIC3 from rat, human, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Kommentare:

Cited Application: IHC

Negative Control: (ABIN7234708)

Blocking Peptide: (ABIN7234708)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu ASIC3

Target: ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))

Andere Bezeichnung: ASIC3

Hintergrund:

Synonyms: Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC

Description: ASIC3 is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC1, ASIC2, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.The functional channel is composed of 4 subunits that can be assembled as homo- or heterotetramers with the other ASIC subunits.A drop in external pH opens the channel resulting in an inward cation current that is quickly inactivated even in the continuous presence of protons in the medium, although a small residual current may persist.Several lines of evidence indicate that ASIC3 may function as a pain sensor. First, it is specifically located in dorsal root ganglia (DRG) neurons and in nociceptors that are involved in pain detection and transmission. In addition, in conditions such as inflammation and cardiac ischemia that include tissue acidosis and thus pain, ASIC3 currents have been detected. Finally, ASIC3 deficient mice display altered sensitivity to high intensity pain produced by heat or acid.Interestingly, while in rats ASIC3 is expressed almost exclusively in DRG neurons, in humans its expression is more widespread suggesting a more extensive role in human nociception.

Gen-ID: 286920

NCBI Accession: NM_004769

UniProt: O35240