ASIC3 Antikörper (Intracellular)

Produktnummer ABIN7042917

Dieses Anti-ASIC3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ASIC3 in WB, IHC, IF und IC. Geeignet für Ratte.

Kurzübersicht für ASIC3 Antikörper (Intracellular) (ABIN7042917)

Target: ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ASIC3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Produktdetails anti-ASIC3 Antikörper

Bindungsspezifität: AA 2-16, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to ASIC3 Channel

Spezifität: Intracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: 15 amino acid residues identical, human - 11,Mouse - 14

Produktmerkmale: Anti-ASIC3 Antibody (ABIN7042917, ABIN7045243 and ABIN7045244) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize ASIC3 from rat, human, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IHC

Negative Control: (ABIN7234708)

Blocking Peptide: (ABIN7234708)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu ASIC3

Target: ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))

Andere Bezeichnung: ASIC3

Hintergrund: Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC,ASIC3 is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC1, ASIC2, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.The functional channel is composed of 4 subunits that can be assembled as homo- or heterotetramers with the other ASIC subunits.A drop in external pH opens the channel resulting in an inward cation current that is quickly inactivated even in the continuous presence of protons in the medium, although a small residual current may persist.Several lines of evidence indicate that ASIC3 may function as a pain sensor. First, it is specifically located in dorsal root ganglia (DRG) neurons and in nociceptors that are involved in pain detection and transmission. In addition, in conditions such as inflammation and cardiac ischemia that include tissue acidosis and thus pain, ASIC3 currents have been detected. Finally, ASIC3 deficient mice display altered sensitivity to high intensity pain produced by heat or acid.Interestingly, while in rats ASIC3 is expressed almost exclusively in DRG neurons, in humans its expression is more widespread suggesting a more extensive role in human nociception.

Alternative names: ASIC3, Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC

Gen-ID: 286920

NCBI Accession: NM_004769

UniProt: O35240