ADRB1 Antikörper (Extracellular)

Produktnummer ABIN7042902

Produktdetails anti-ADRB1 Antikörper

Target: ADRB1 (Adrenergic, beta-1-, Receptor (ADRB1))

Bindungsspezifität: AA 200-214, Extracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADRB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to β1-Adrenergic Receptor

Spezifität: 2nd extracellular loop

Kreuzreaktivität: Maus, Ratte

Homologie: Rat,Mouse,human - 14,15 amino acid residues identical

Produktmerkmale: Anti-β1-Adrenergic Receptor (extracellular) Antibody (ABIN7042902, ABIN7043924 and ABIN7043925)) is a highly specific antibody directed against an extracellular epitope the rat β1-adrenoceptor. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize β1AR from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RAESDEARR(S)YNDP, corresponding to amino acid residues 200-214 of rat ADRB1 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: BLP-AR023

Blocking Peptide: BLP-AR023

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu ADRB1

Target: ADRB1 (Adrenergic, beta-1-, Receptor (ADRB1))

Andere Bezeichnung: ADRB1 (ADRB1 Produkte)

Hintergrund: Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR,β-Adrenergic receptors (βAR) belong to the superfamily of G-protein coupled receptors (GPCRs). βAR stimulation by the sympathetic nervous system or circulating catecholamines is involved in peripheral blood circulation, metabolic regulation, muscle contraction, and central neural activities. In the heart, acute βAR stimulation serves as the most powerful means to regulate cardiac output in response to a fight-or-flight situation.There are three βAR subtypes, β1AR, β2AR and β3AR, in cardiac myocytes1. The receptors consist of seven membrane-spanning domains, three intra- and three extracellular loops, an extracellular N-terminal domain, and an intracellular C-terminal tail2.β1AR is the predominant receptor subtype expressed in human cardiomyocytes. β1AR is also expressed in the kidney3. It has been implicated in adipogenesis and memory formation4.β1AR has been widely connected with heart disease. Both the downregulation and overstimulation of β1AR have been connected to myocyte apoptosis. In addition, chronic heart failure is characterized by abnormalities in β1AR signal transduction. It was demonstrated that heart failure is also inducible in animal models by overexpression of components of the β1AR signal transduction cascade. Transgenic mice overexpressing β1AR develop, with aging, heart failure similar to that observed in dilated cardiomyopathy in humans5.

Alternative names: beta1-Adrenergic Receptor, Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR

Gen-ID: 64508

NCBI Accession: NM_000684

UniProt: P18090

Pathways: cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Brown Fat Cell Differentiation