ADCYAP1R1 Antikörper (Intracellular)

Produktnummer ABIN7042894

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ADCYAP1R1 in WB, IHC und IF. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für ADCYAP1R1 Antikörper (Intracellular) (ABIN7042894)

Target: ADCYAP1R1 (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) Receptor Type I (ADCYAP1R1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADCYAP1R1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: KO Validated

Produktdetails anti-ADCYAP1R1 Antikörper

Bindungsspezifität: AA 474-487, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to PACAP Type I Receptor

Spezifität: Intracellular, C-terminus

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 12,14 amino acid residues identical

Produktmerkmale: Anti-PACAP Receptor 1 (PAC1) Antibody is directed against an epitope of the rat PACAP type I receptor. Anti-PACAP Receptor 1 (PAC1) Antibody (ABIN7042894, ABIN7045333 and ABIN7045334) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PAC1 receptor from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KVNRYFTMDFKHRH, corresponding to amino acid residues 474-487 of rat PAC1 receptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:500

Kommentare:

Cited Application: IHC

Negative Control: (ABIN7234650)

Blocking Peptide: (ABIN7234650)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu ADCYAP1R1

Target: ADCYAP1R1 (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) Receptor Type I (ADCYAP1R1))

Andere Bezeichnung: ADCYAP1R1

Hintergrund: PACAP type I receptor, Pituitary adenylate cyclase-activating polypeptide type I receptor, PACAP-R1, ADCYAP1R1,Pituitary adenylate cyclase-activating polypeptide (PACAP, encoded by ADCYAP1) belongs a superfamily of structurally related peptide hormones, which include vasoactive intestinal peptide (VIP), glucagon, glucagon-like peptides, secretin, gastric inhibitory peptide and growth hormone-releasing hormone.PACAP acts through PAC1, VPAC1 and VPAC2, initiating multiple signaling pathways1. PAC1 is a class B member of the G-protein coupled receptors (GPCRs) superfamily. Class B receptors are differentiated from Class A by a relatively large N-terminal ectodomain that is crucial to ligand recognition2.PAC1R is mainly expressed on smooth muscle cells, neurons, endothelial cells and peritoneal macrophages3.PAC1R is associated with posttraumatic stress disorder (PTSD) symptoms and psychophysiological markers of fear and anxiety4.PAC1 receptor null mice demonstrate reduced anxiety-like behavior5. In addition, PAC1 overexpression can result in hydrocephalus- and schizophrenia-related neuropathology1.

Alternative names: PACAP Receptor 1 (PAC1), PACAP type I receptor, Pituitary adenylate cyclase-activating polypeptide type I receptor, PACAP-R1, ADCYAP1R1

Gen-ID: 24167

NCBI Accession: NM_001118

UniProt: P32215

Pathways: Neurotrophin Signalübertragung, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process