ADCYAP1R1 Antikörper (C-Term)

Produktnummer ABIN1048299

Produktdetails anti-ADCYAP1R1 Antikörper

Target: ADCYAP1R1 (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) Receptor Type I (ADCYAP1R1))

Bindungsspezifität: C-Term

Reaktivität: Human, Affe, Hund, Huhn, Xenopus laevis, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADCYAP1R1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human ADCYAP1R1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Bat, Chicken, Lizard, Xenopus (100%) Stickleback, Pufferfish, Zebrafish (94%) Mouse, Rat, Hamster, Elephant, Bovine, Horse, Pig (88%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 17 amino acid peptide from C-terminus of human ADCYAP1R1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Bat, Chicken, Lizard, Xenopus (100%), Stickleback, Pufferfish, Zebrafish (94%), Mouse, Rat, Hamster, Elephant, Bovine, Horse, Pig (88%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (15 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu ADCYAP1R1

Target: ADCYAP1R1 (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) Receptor Type I (ADCYAP1R1))

Andere Bezeichnung: ADCYAP1R1 / PAC1 Receptor (ADCYAP1R1 Produkte)

Synonyme: PAC1 antikoerper, PAC1R antikoerper, PACAPR antikoerper, PACAPRI antikoerper, 2900024I10Rik antikoerper, AI846590 antikoerper, PACAP1-R antikoerper, pac1 antikoerper, pacapr antikoerper, pacapri antikoerper, PAC1-R antikoerper, PACAP-R1 antikoerper, PACAP-R1A antikoerper, PACAPR1 antikoerper, PACAPR1A antikoerper, adcyap1r1 antikoerper, adcyap1r1-A antikoerper, ADCYAP1R1 antikoerper, ADCYAP receptor type I antikoerper, adenylate cyclase activating polypeptide 1 receptor 1 antikoerper, adenylate cyclase activating polypeptide 1 (pituitary) receptor type I antikoerper, ADCYAP receptor type 1 antikoerper, adenylate cyclase activating polypeptide 1a (pituitary) receptor type I antikoerper, adenylate cyclase activating polypeptide 1 (pituitary) receptor type I S homeolog antikoerper, ADCYAP1R1 antikoerper, Adcyap1r1 antikoerper, adcyap1r1 antikoerper, adcyap1r1a antikoerper, adcyap1r1.S antikoerper

Hintergrund: Name/Gene ID: ADCYAP1R1
Subfamily: Vasoactive intestinal polypeptide
Family: GPCR

Synonyms: ADCYAP1R1, PAC1, Pac1 receptor, Pac1 receptors, PAC1-R, PACAP receptor 1, Pacap specific receptor, PACAP-R-1, PACAP/VIP receptors, PACAP1 receptor, PACAP Receptor, Pacap receptor type 1, Pacap type 1 receptor, PACAP type I receptor, PACAPR, PAC1R, PACAP-R1, PACAPRI, Pvr1, Pacap receptor type i

Gen-ID: 117

Pathways: Neurotrophin Signalübertragung, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process