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CD73 Antikörper (Extracellular)

Der Kaninchen Polyklonal Anti-CD73-Antikörper wurde für WB, IHC, FACS, IF und LCI validiert. Er ist geeignet, CD73 in Proben von Maus zu detektieren.
Produktnummer ABIN7042877

Kurzübersicht für CD73 Antikörper (Extracellular) (ABIN7042877)

Target

Alle CD73 (NT5E) Antikörper anzeigen
CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reaktivität

  • 186
  • 77
  • 37
  • 19
  • 18
  • 16
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Maus

Wirt

  • 107
  • 99
  • 22
  • 2
  • 2
  • 1
Kaninchen

Klonalität

  • 138
  • 94
  • 1
Polyklonal

Konjugat

  • 100
  • 21
  • 17
  • 9
  • 8
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser CD73 Antikörper ist unkonjugiert

Applikation

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  • 27
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  • 14
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Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)
  • Bindungsspezifität

    • 16
    • 13
    • 9
    • 9
    • 9
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
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    • 1
    AA 429-433, Extracellular

    Verwendungszweck

    A Rabbit Polyclonal Antibody to CD73

    Spezifität

    Extracellular, N-terminus

    Kreuzreaktivität

    Human, Maus, Ratte

    Homologie

    Human,rat - identical

    Produktmerkmale

    Anti-CD73 (extracellular) Antibody (ABIN7042877, ABIN7044811 and ABIN7044812) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize NT5E from human, rat and mouse samples.

    Aufreinigung

    Affinity purified on immobilized antigen.

    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)KGSTLKKAFEHSVHR, corresponding to amino acid residues 429-433 of mouse CD73

    Isotyp

    IgG
  • Applikationshinweise

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

    Application Dilutions Western blot wb: 1:200

    Kommentare

    Negative Control: (ABIN7234599)

    Blocking Peptide: (ABIN7234599)

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

    Konzentration

    1 mg/mL

    Buffer

    PBS pH 7.4

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target

    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

    Andere Bezeichnung

    NT5E

    Hintergrund

    Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E,CD73 (ecto-5'-nucleotidase, NT5E) is a glycosylphosphatidylinositol enzyme expressed on the cell surface of various cell types. CD73 is responsible for hydrolyzing extracellular adenosine monophosphate to adenosine and inorganic phosphate, which results in activation of the G-protein coupled receptors, namely A2AR and A2BR, to exert potent immunoregulatory activity.CD73 plays an important role in homeostatic functions in healthy organs and tissues. The protein is involved as an inhibitory immune checkpoint molecule, it promotes immune escape of tumor cells since free adenosine generated by the protein inhibits cellular immune responses1,2.CD73 primarily detected in cancer cells and in immune cells such as CD4+Foxp3+ regulatory T cells, and myeloid-derived suppressor cells that are recruited by the tumor2.Studies show that CD73 expression is significantly up-regulated in numerous malignant tumors such as breast cancer, colorectal cancer, prostate cancer, ovarian cancer, and gallbladder cancer3.

    Alternative names: CD73, Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E

    Gen-ID

    23959

    NCBI Accession

    NM_001204813

    UniProt

    Q61503

    Pathways

    Synaptic Membrane, Ribonucleoside Biosynthetic Process
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