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CD73 Antikörper (Extracellular)

NT5E Reaktivität: Maus WB, FACS, IHC, IF, LCI Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN7042877
  • Target Alle CD73 (NT5E) Antikörper anzeigen
    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
    Bindungsspezifität
    • 15
    • 9
    • 9
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 429-433, Extracellular
    Reaktivität
    • 113
    • 68
    • 27
    • 17
    • 16
    • 15
    • 3
    • 1
    • 1
    • 1
    Maus
    Wirt
    • 82
    • 41
    • 21
    • 2
    • 1
    Kaninchen
    Klonalität
    • 75
    • 71
    • 1
    Polyklonal
    Konjugat
    • 58
    • 13
    • 11
    • 8
    • 8
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD73 Antikörper ist unkonjugiert
    Applikation
    • 75
    • 72
    • 42
    • 42
    • 15
    • 13
    • 13
    • 13
    • 12
    • 8
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI)
    Verwendungszweck
    A Rabbit Polyclonal Antibody to CD73
    Spezifität
    Extracellular, N-terminus
    Kreuzreaktivität
    Human, Maus, Ratte
    Homologie
    Human,rat - identical
    Produktmerkmale
    Anti-CD73 (extracellular) Antibody (ABIN7042877, ABIN7044811 and ABIN7044812) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize NT5E from human, rat and mouse samples.
    Aufreinigung
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)KGSTLKKAFEHSVHR, corresponding to amino acid residues 429-433 of mouse CD73

    Isotyp
    IgG
    Top Product
    Discover our top product NT5E Primärantikörper
  • Applikationshinweise

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

    Application Dilutions Western blot wb: 1:200

    Kommentare

    Negative Control: (ABIN7234599)

    Blocking Peptide: (ABIN7234599)

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
    Andere Bezeichnung
    NT5E (NT5E Produkte)
    Hintergrund
    Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E,CD73 (ecto-5'-nucleotidase, NT5E) is a glycosylphosphatidylinositol enzyme expressed on the cell surface of various cell types. CD73 is responsible for hydrolyzing extracellular adenosine monophosphate to adenosine and inorganic phosphate, which results in activation of the G-protein coupled receptors, namely A2AR and A2BR, to exert potent immunoregulatory activity.CD73 plays an important role in homeostatic functions in healthy organs and tissues. The protein is involved as an inhibitory immune checkpoint molecule, it promotes immune escape of tumor cells since free adenosine generated by the protein inhibits cellular immune responses1,2.CD73 primarily detected in cancer cells and in immune cells such as CD4+Foxp3+ regulatory T cells, and myeloid-derived suppressor cells that are recruited by the tumor2.Studies show that CD73 expression is significantly up-regulated in numerous malignant tumors such as breast cancer, colorectal cancer, prostate cancer, ovarian cancer, and gallbladder cancer3.

    Alternative names: CD73, Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E
    Gen-ID
    23959
    NCBI Accession
    NM_001204813
    UniProt
    Q61503
    Pathways
    Synaptic Membrane, Ribonucleoside Biosynthetic Process
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