Cathepsin B Antikörper (Internal Region)

Produktnummer ABIN7013865

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Cathepsin B in WB, IHC, ELISA, FACS und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Cathepsin B Antikörper (Internal Region) (ABIN7013865)

Target: Cathepsin B (CTSB)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Cathepsin B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-Cathepsin B Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Cathepsin B

Sequenz: SDRICIHTNA HVSVE

Spezifität: Reported variants represent identical protein: NP_680091.1, NP_680090.1, NP_680092.1, NP_001899.1, NP_680093.1.

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.

Western Blot: Approx 35 kDa band observed in lysates of cell line HepG2 and in Human Lung, Human Colo-rectal carcinoma and Human Brain Glio-Blastoma cancer lysates (calculated MW of 37.8x kDa according to NP_001899.1). Recommended concentration: 0.5-2 μg/m

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ vesicles of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Cathepsin B

Target: Cathepsin B (CTSB)

Andere Bezeichnung: CTSB

Hintergrund: CTSB, cathepsin B, APPS, CPSB, APP secretase, amyloid precursor protein secretase, cathepsin B1, cysteine protease

Gen-ID: 1508

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades