Deoxyuridine Triphosphatase (DUT) Antikörper

Produktnummer ABIN7260715

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch in WB, IHC und IF. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.

Kurzübersicht für Deoxyuridine Triphosphatase (DUT) Antikörper (ABIN7260715)

Target: Deoxyuridine Triphosphatase (DUT)

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails

Produktmerkmale: Polyclonal Antibody

Aufreinigung: Affinity purification

Immunogen: Recombinant fusion protein of human DUT (NP_001020419.1).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000 IHC 1:50-1:200 IF 1:100-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Antigendetails

Target: Deoxyuridine Triphosphatase (DUT)

Andere Bezeichnung: DUT

Hintergrund: This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

Molekulargewicht:

Observed_MW: 24 kDa

Calculated_MW: 17 kDa/26 kDa

Gen-ID: 1854

UniProt: P33316