GFP Tag Antikörper

Produktnummer ABIN7250878

Produktdetails anti-GFP Tag Antikörper

Target: GFP Tag

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFP Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktmerkmale: Tag Antibody

Aufreinigung: Affinity purification

Immunogen: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:5000-1:10000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.4.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Details zu GFP Tag

Target: GFP Tag

Andere Bezeichnung: GFP-Tag (GFP Tag Produkte)

Synonyme: green fluorescent protein antikoerper, gfp antikoerper

Substanzklasse: Tag

Hintergrund: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9 kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em).