GFP Tag Antikörper (N-Term)

Produktnummer ABIN2443932

Der Maus Monoklonal anti-GFP Tag Antikörper (Klon GF28R) (ABIN2443932) detektiert spezifisch GFP Tag in WB, ELISA, IHC, IP und DB. Dieser Antikörper reagiert spezifisch mit Proben aus Aequorea victoria.

Kurzübersicht für GFP Tag Antikörper (N-Term) (ABIN2443932)

Target: GFP Tag

Reaktivität: Aequorea victoria

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GFP Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Dot Blot (DB)

Klon: GF28R

Produktdetails anti-GFP Tag Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: GFP Tag Mouse Monoclonal Antibody (GF28R)

Spezifität: The antibody recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP.

Aufreinigung: Protein G purified

Immunogen: GFP from the jellyfish Aequorea victoria N-terminal peptide-KLH conjugates.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB 1:1000,IHC 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Details zu GFP Tag

Target: GFP Tag

Substanzklasse: Tag

Hintergrund: Epitope tags have application in the labeling, isolation and detection of proteins using immunoblotting, Immunoprecipitation and immunostaining techniques. The green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is involved in the bioluminescence of the jellyfish. When expressed in prokaryotic cells, GFP produces a fluorescent product, making GFP useful in monitoring physiological processes, visualizing protein localization, and detecting transgenic expression.

UniProt: P42212