PRKAA1/PRKAA2 Antikörper (pThr172, pThr183)

Produktnummer ABIN7250794

Dieses Anti-PRKAA1/PRKAA2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PRKAA1/PRKAA2 in WB, ELISA, IHC (p) und IF. Geeignet für Human, Maus, Ratte und Affe.

Kurzübersicht für PRKAA1/PRKAA2 Antikörper (pThr172, pThr183) (ABIN7250794)

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKAA1/PRKAA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-PRKAA1/PRKAA2 Antikörper

Bindungsspezifität: pThr172, pThr183

Produktmerkmale: Phosphorylated antibody

Aufreinigung: Affinity purification

Immunogen: Synthesized peptide derived from human AMPKalpha1/2 around the phosphorylation site of Thr183/172

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol, pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Details zu PRKAA1/PRKAA2

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Andere Bezeichnung: AMPK alpha1/2

Hintergrund: AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively.

Molekulargewicht:

Observed_MW: 63 kDa

Calculated_MW: 62 kDa

UniProt: Q13131, P54646