PRKAA1/PRKAA2 Antikörper (pThr172, pThr183)

Produktnummer ABIN7201115

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PRKAA1/PRKAA2 in WB, IHC, IF und ELISA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Schwein, Affe und Penaeus japonicus.

Kurzübersicht für PRKAA1/PRKAA2 Antikörper (pThr172, pThr183) (ABIN7201115)

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Reaktivität: Human, Maus, Ratte, Schwein, Affe, Penaeus japonicus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKAA1/PRKAA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Produktdetails anti-PRKAA1/PRKAA2 Antikörper

Bindungsspezifität: pThr172, pThr183

Verwendungszweck: Rabbit Anti-AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody

Spezifität: Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein only when phosphorylated at T183/172.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human AMPKalpha1/2 Phospho-Thr183/172

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200,WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,Not yet tested in other applications,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu PRKAA1/PRKAA2

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Andere Bezeichnung: AMPKalpha1/2

Hintergrund: PRKAA1, AMPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, PRKAA2, AMPK,Protein kinase AMP-activated catalytic subunit alpha 1 encoded by PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Molekulargewicht: 63kD

Gen-ID: 5562, 5563

UniProt: Q13131, P54646