ARNT Antikörper

Produktnummer ABIN7250482

Produktdetails anti-ARNT Antikörper

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ARNT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktmerkmale: Monoclonal Antibody

Aufreinigung: Protein A purification

Immunogen: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:1000-2000, IHC 1:100-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol, pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Details zu ARNT

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Andere Bezeichnung: HIF1 beta (ARNT Produkte)

Synonyme: HIF-1-beta antikoerper, HIF-1beta antikoerper, HIF1-beta antikoerper, HIF1B antikoerper, HIF1BETA antikoerper, TANGO antikoerper, bHLHe2 antikoerper, D3Ertd557e antikoerper, Drnt antikoerper, ESTM42 antikoerper, Hif1b antikoerper, W08714 antikoerper, mKIAA4051 antikoerper, Arnt1 antikoerper, ARNT antikoerper, zgc:136664 antikoerper, arnt1 antikoerper, hif1b antikoerper, tango antikoerper, xArnt antikoerper, hif1beta antikoerper, hif-1beta antikoerper, arnt.2 antikoerper, MGC68805 antikoerper, aryl hydrocarbon receptor nuclear translocator antikoerper, aryl hydrocarbon receptor nuclear translocator S homeolog antikoerper, ARNT antikoerper, Arnt antikoerper, arnt antikoerper, arnt.S antikoerper

Hintergrund: Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex, ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8).

Molekulargewicht: 87 kDa

UniProt: P27540

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effekt