Deoxyuridine Triphosphatase (DUT) Antikörper

Produktnummer ABIN7237970

Der Kaninchen Polyklonal Anti--Antikörper wurde für WB, IHC und ELISA validiert. Er ist geeignet, in Proben von Human und Ratte zu detektieren.

Kurzübersicht für Deoxyuridine Triphosphatase (DUT) Antikörper (ABIN7237970)

Target: Deoxyuridine Triphosphatase (DUT)

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails

Produktmerkmale: Polyclonal Antibody

Aufreinigung: Affinity purification

Immunogen: Synthetic peptide of human DUT

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:200-1:1000, IHC 1:10-1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.05 % sodium azide and 50 % glycerol, PH7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Antigendetails

Target: Deoxyuridine Triphosphatase (DUT)

Andere Bezeichnung: DUT

Hintergrund: This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

Molekulargewicht: 27 kDa

NCBI Accession: NP_001939

UniProt: P33316