GAPDH Antikörper (AA 2-148)

Produktnummer ABIN7429394

Der Kaninchen Polyklonal Anti-GAPDH-Antikörper wurde für WB, IHC, IP und ICC validiert. Er ist geeignet, GAPDH in Proben von Human zu detektieren.

Kurzübersicht für GAPDH Antikörper (AA 2-148) (ABIN7429394)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAPDH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-GAPDH Antikörper

Bindungsspezifität: AA 2-148

Verwendungszweck: Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)

Spezifität: The antibody is a rabbit polyclonal antibody raised against GAPDH. It has been selected for its ability to recognize GAPDH in immunohistochemical staining and western blotting.

Kreuzreaktivität: Huhn, Rind (Kuh), Hund, Ziege, Meerschweinchen, Pferd, Maus, Schwein, Ratte

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) corresdonding to Gly2~Ser148 with N-terminal His Tag

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung: GAPDH

Hintergrund: G3PD, GAPD, Peptidyl-cysteine S-nitrosylase GAPDH

UniProt: P04406