ube3a Antikörper (AA 158-407)

Produktnummer ABIN7433114

Der Kaninchen Polyklonal Anti-ube3a-Antikörper wurde für WB, IHC, IP und ICC validiert. Er ist geeignet, ube3a in Proben von Human zu detektieren.

Kurzübersicht für ube3a Antikörper (AA 158-407) (ABIN7433114)

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ube3a Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-ube3a Antikörper

Bindungsspezifität: AA 158-407

Verwendungszweck: Polyclonal Antibody to Ubiquitin Protein Ligase E3A (UBE3A)

Spezifität: The antibody is a rabbit polyclonal antibody raised against UBE3A. It has been selected for its ability to recognize UBE3A in immunohistochemical staining and western blotting.

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Ubiquitin Protein Ligase E3A (UBE3A) corresdonding to Ser158~Leu407 with N-terminal His Tag

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1-5 μg/mLImmunocytochemistry in formalin fixed cells: 5-20 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-20 μg/mLImmunohistochemistry in paraffin section: 5-20 μg/mLEnzyme-linked Immunosorbent Assay: 0.05-2 μg/mLOptimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu ube3a

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Andere Bezeichnung: UBE3A

Hintergrund: ANCR, AS, E6-AP, EPVE6AP, HPVE6A, Papilloma Virus E6-Associated,Angelman Syndrome, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway