LSD1 Antikörper (N-Term)

Produktnummer ABIN6992152

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch LSD1 in WB, ELISA und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für LSD1 Antikörper (N-Term) (ABIN6992152)

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LSD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-LSD1 Antikörper

Bindungsspezifität: AA 90-140, N-Term

Spezifität: At least two isoforms of LSD1 are known to exist, this antibody detects both.

Aufreinigung: LSD1 antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-LSD1 antibody was raised against a peptide corresponding to 19 amino acids near the amino terminus of human LSD1. The immunogen is located within amino acids 90-140 of LSD1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1 - 2 μ,g/mL, IHC-P: 5 μ,g/mL.

Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: LSD1 antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: LSD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu LSD1

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Andere Bezeichnung: LSD1

Hintergrund: Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction (1). Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription (2). LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation (3,4). Experiments have shown that CoREST, a SANT domain-containing corepressor (5) acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein (6), inhibits CoREST/LSD1 activity in vitro (3). LSD1-mediated histone demethylation thus may have significant effects on gene expression.

Molekulargewicht: Predicted: 93kD

Observed: 93 kD kDa

Gen-ID: 23028

NCBI Accession: NP_001009999

UniProt: O60341

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, Warburg Effekt