ARID1A Antikörper (N-Term)

Produktnummer ABIN6992134

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ARID1A in ELISA, WB, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für ARID1A Antikörper (N-Term) (ABIN6992134)

Target: ARID1A (AT Rich Interactive Domain 1A (SWI-Like) (ARID1A))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ARID1A Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ARID1A Antikörper

Bindungsspezifität: AA 580-630, N-Term

Spezifität: ARID1A antibody is human, mouse and rat reactive. Multiple isoforms of ARID1A protein are known to exist.

Aufreinigung: ARID1A antibody is affinity chromatography purified via peptide column.

Immunogen: ARID1A antibody was raised against an 18 amino acid peptide near the amino terminus of human ARID1A. The immunogen is located within amino acids 580 - 630 of ARID1A.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ARID1A antibody can be used for detection of ARID1A by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: ARID1A antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: ARID1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu ARID1A

Target: ARID1A (AT Rich Interactive Domain 1A (SWI-Like) (ARID1A))

Andere Bezeichnung: ARID1A

Hintergrund: The ARID1A protein is a member of the SWI/SNF family, whose members are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. ARID1A is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (1). It possesses a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. The C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that ARID1A confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions (2).

Molekulargewicht: Predicted: 133, 227, 251 kDa

Observed: 130, 260 kDa

Gen-ID: 8289

NCBI Accession: NP_006006

UniProt: O14497

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Tube Formation