CRBN Antikörper (C-Term)

Produktnummer ABIN6992111

Der Kaninchen Polyklonal Anti-CRBN-Antikörper wurde für WB, ELISA, IF und IHC (p) validiert. Er ist geeignet, CRBN in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für CRBN Antikörper (C-Term) (ABIN6992111)

Target: CRBN (Cereblon (CRBN))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CRBN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CRBN Antikörper

Bindungsspezifität: C-Term

Spezifität: CRBN antibody is human, mouse and rat reactive. At least two isoforms of CRBN are known to exist, this antibody will detect both isoforms.

Aufreinigung: CRBN antibody is affinity chromatography purified via peptide column.

Immunogen: CRBN antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CRBN. The immunogen is located within the last 50 amino acids of CRBN.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: CRBN antibody can be used for detection of CRBN by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: CRBN antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: CRBN antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu CRBN

Target: CRBN (Cereblon (CRBN))

Andere Bezeichnung: CRBN

Hintergrund: CRBN, a member of the Lon protease protein family, plays a role in brain development (1). It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8 (2). CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain (3,4).

Molekulargewicht: Predicted: 49 kDa

Observed: 54 kDa

Gen-ID: 51185

NCBI Accession: NP_057386

UniProt: Q96SW2