ATG4B Antikörper (C-Term)

Produktnummer ABIN6992096

Der Kaninchen Polyklonal Anti-ATG4B-Antikörper wurde für WB, ELISA, IHC (p) und IF validiert. Er ist geeignet, ATG4B in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für ATG4B Antikörper (C-Term) (ABIN6992096)

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG4B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-ATG4B Antikörper

Bindungsspezifität: C-Term

Spezifität: ATG4B antibody is human, mouse and rat reactive. At least two isoforms of ATG4B are known to exist, this antibody will detect only the larger isoform. ATG4B is predicted to not cross-react with other ATG4 proteins.

Aufreinigung: ATG4B antibody is affinity chromatography purified via peptide column.

Immunogen: ATG4B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ATG4B. The immunogen is located within the last 50 amino acids of ATG4B.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ATG4B antibody can be used for detection of ATG4B by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: ATG4B antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: ATG4B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu ATG4B

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Andere Bezeichnung: ATG4B

Hintergrund: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4B, also known as AUTL1, is one of four mammalian orthologs of the yeast ATG4 protein, all four are cysteine proteases (3). ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4). ATG4B has been found to be a novel protective protein in inflammatory colitis (5).

Molekulargewicht: Predicted: 43 kDa

Observed: 46 kDa

Gen-ID: 23192

NCBI Accession: NP_037457

UniProt: Q9Y4P1

Pathways: Autophagie