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LIMA1 Antikörper (N-Term)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch LIMA1 in WB, ELISA, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human.
Produktnummer ABIN6992089

Kurzübersicht für LIMA1 Antikörper (N-Term) (ABIN6992089)

Target

Alle LIMA1 Antikörper anzeigen
LIMA1 (LIM Domain and Actin Binding 1 (LIMA1))

Reaktivität

  • 32
  • 8
  • 7
  • 6
  • 6
  • 5
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 26
  • 6
Kaninchen

Klonalität

  • 26
  • 6
Polyklonal

Konjugat

  • 26
  • 2
  • 1
  • 1
  • 1
  • 1
Dieser LIMA1 Antikörper ist unkonjugiert

Applikation

  • 31
  • 15
  • 9
  • 6
  • 5
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Bindungsspezifität

    • 8
    • 5
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 230-280, N-Term

    Spezifität

    LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist, this antibody will only detect the three largest isoforms.

    Aufreinigung

    LIMA1 antibody is affinity chromatography purified via peptide column.

    Immunogen

    LIMA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human LIMA1. The immunogen is located within amino acids 230 - 280 of LIMA1.

    Isotyp

    IgG
  • Applikationshinweise

    LIMA1 antibody can be used for detection of LIMA1 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

    Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    LIMA1 antibody is supplied in PBS containing 0.02 % sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    LIMA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
  • Target

    LIMA1 (LIM Domain and Actin Binding 1 (LIMA1))

    Andere Bezeichnung

    LIMA1

    Hintergrund

    The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking the cadherin-catenin complex to the actin cytoskeleton (3). Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4).

    Molekulargewicht

    Predicted: 50, 66, 84 kDa

    Observed: 65 kDa

    Gen-ID

    51474

    NCBI Accession

    NP_001107018

    UniProt

    Q9UHB6
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