LIMA1 Antikörper (N-Term)
Kurzübersicht für LIMA1 Antikörper (N-Term) (ABIN6992089)
Target
Alle LIMA1 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 230-280, N-Term
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Spezifität
- LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist, this antibody will only detect the three largest isoforms.
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Aufreinigung
- LIMA1 antibody is affinity chromatography purified via peptide column.
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Immunogen
- LIMA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human LIMA1. The immunogen is located within amino acids 230 - 280 of LIMA1.
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Isotyp
- IgG
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Applikationshinweise
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LIMA1 antibody can be used for detection of LIMA1 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- LIMA1 antibody is supplied in PBS containing 0.02 % sodium azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- -20 °C,4 °C
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Informationen zur Lagerung
- LIMA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
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- LIMA1 (LIM Domain and Actin Binding 1 (LIMA1))
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Andere Bezeichnung
- LIMA1
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Hintergrund
- The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking the cadherin-catenin complex to the actin cytoskeleton (3). Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4).
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Molekulargewicht
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Predicted: 50, 66, 84 kDa
Observed: 65 kDa -
Gen-ID
- 51474
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NCBI Accession
- NP_001107018
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UniProt
- Q9UHB6
Target
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