E2F3 Antikörper (AA 150-200)

Produktnummer ABIN6992046

Dieses Anti-E2F3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von E2F3 in WB, ELISA, IHC (p) und IF. Geeignet für Human und Maus.

Kurzübersicht für E2F3 Antikörper (AA 150-200) (ABIN6992046)

Target: E2F3

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-E2F3 Antikörper

Bindungsspezifität: AA 150-200

Spezifität: E2F3 antibody is human and mouse reactive. At least three isoforms of E2F3 are known to exist, this antibody will detect only the largest isoform. This antibody is predicted to not cross-react with other members of the E2F transcription factor family.

Aufreinigung: E2F3 antibody is affinity chromatography purified via peptide column.

Immunogen: E2F3 antibody was raised against a 15 amino acid peptide near the center of human E2F3. The immunogen is located within amino acids 150 - 200 of E2F3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: E2F3 antibody can be used for detection of E2F3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: E2F3 antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: E2F3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu E2F3

Target: E2F3

Hintergrund: The E2F transcription factor 3 (E2F3) is a member of a small family of transcription factors that function through binding of DP interaction partner proteins. E2F3 recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle (1). Like the related E2F1 and E2F2, E2F3 is essential for cellular proliferation and progression through the cell cycle (2). Altered copy number and activity of this gene have been observed in a number of human cancers (3).

Molekulargewicht: Predicted: 51 kDa

Observed: 56 kDa

Gen-ID: 1871

NCBI Accession: NP_001940

UniProt: O00716