E2F3 Antikörper (C-Term)

Produktnummer ABIN2780388

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-E2F3 Antikörper wird verwendet zum Nachweis von E2F3 in Proben von Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd und Kaninchen. Er wurde validiert für WB.

Kurzübersicht für E2F3 Antikörper (C-Term) (ABIN2780388)

Target: E2F3

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-E2F3 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: E2F3 Antibody - C-terminal region

Sequenz: LLQQTEDQIP SNLEGPFVNL LPPLLQEDYL LSLGEEEGIS DLFDAYDLEK

Spezifität: The immunizing peptide used to raise this antibody is 100 % homologous to isoform 2 (334aa, 37 kDa) of human E2F3.

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 92%, Rat: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against E2F3. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human E2F3

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38089-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-E2F3 Antikörper (ABIN2780388)

Giangreco, Vaishnav, Wagner, Finelli, Fleshner, Van der Kwast, Vieth, Nonn: "Tumor suppressor microRNAs, miR-100 and -125b, are regulated by 1,25-dihydroxyvitamin D in primary prostate cells and in patient tissue." in: Cancer prevention research (Philadelphia, Pa.), Vol. 6, Issue 5, pp. 483-94, (2013) (PubMed).

Melcher, Hartmann, Zopf, Herterich, Wilke, Müller, Rosler, Kudlich, Al-Taie, Rosenwald, Katzenberger, Scholtka, Seibold, Rogoll, Scheppach, Scheurlen, Lührs: "LOH and copy neutral LOH (cnLOH) act as alternative mechanism in sporadic colorectal cancers with chromosomal and microsatellite instability." in: Carcinogenesis, Vol. 32, Issue 4, pp. 636-42, (2011) (PubMed).

Naga Prasad, Duan, Gupta, Surampudi, Volinia, Calin, Liu, Kotwal, Moravec, Starling, Perez, Sen, Wu, Plow, Croce, Karnik: "Unique microRNA profile in end-stage heart failure indicates alterations in specific cardiovascular signaling networks." in: The Journal of biological chemistry, Vol. 284, Issue 40, pp. 27487-99, (2009) (PubMed).

Details zu E2F3

Target: E2F3

Hintergrund:

Background Information: E2F3 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. E2F3 binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.

Gene Name: E2F transcription factor 3

Alternative Symbols: E2F-3

Protein Name: Putative uncharacterized protein DKFZp686C18211 EMBL CAH18140.1

Molekulargewicht: 49 kDa

Gen-ID: 1871

UniProt: Q68DT0