HMGB1 Antikörper (AA 70-120)

Produktnummer ABIN6991976

Der Kaninchen Polyklonal anti-HMGB1 Antikörper wird verwendet zum Nachweis von HMGB1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IF und IHC (p).

Kurzübersicht für HMGB1 Antikörper (AA 70-120) (ABIN6991976)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HMGB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HMGB1 Antikörper

Bindungsspezifität: AA 70-120

Spezifität: HMGB1 antibody is human, mouse and rat reactive.

Aufreinigung: HMGB1 antibody is affinity chromatography purified via peptide column.

Immunogen: HMGB1 antibody was raised against a 19 amino acid peptide near the center of human HMGB1. The immunogen is located within amino acids 70 - 120 of HMGB1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: HMGB1 antibody can be used for detection of HMGB1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: HMGB1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: HMGB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Andere Bezeichnung: HMGB1

Hintergrund: The high-mobility group box 1 (HMGB1) protein was originally identified as a highly conserved nuclear DNA-binding protein that participates in DNA replication, repair and transcriptional regulation of gene expression (1,2). However, HMGB1 has more recently emerged as an extracellularly released mediator of inflammation and repair responses in lipopolysaccharide (LPS)-induced endotoxemia and sepsis (3). HMGB1 has also been suggested to induce secretion of pro-inflammatory cytokines and chemokines by inducing NF-kB signaling downstream of Toll-like receptor (TLR) 2 and TLR4 activation (4).

Molekulargewicht: Predicted: 24 kDa

Observed: 24 kDa

Gen-ID: 3146

NCBI Accession: NP_002119

UniProt: P09429

Pathways: p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome