ITCH Antikörper (N-Term)

Produktnummer ABIN6991954

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ITCH in ELISA und WB. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für ITCH Antikörper (N-Term) (ABIN6991954)

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ITCH Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Produktdetails anti-ITCH Antikörper

Bindungsspezifität: AA 40-90, N-Term

Spezifität: ITCH antibody is human, mouse and rat reactive. At least three isoforms of ITCH are known to exist, this antibody only recognizes the two longest isoforms. This antibody is predicted to not cross-react with other members of the Nedd4 protein family.

Aufreinigung: ITCH antibody is affinity chromatography purified via peptide column.

Immunogen: ITCH antibody was raised against a 15 amino acid peptide near the amino terminus of human ITCH . The immunogen is located within amino acids 40 - 90 of ITCH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ITCH antibody can be used for detection of ITCH by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: ITCH antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: ITCH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Andere Bezeichnung: ITCH

Hintergrund: The Itchy E3 ubiquitin protein ligase (ITCH) is a member of the Nedd4 family of HECT domain E3 ubiquitin ligases (1). HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. ITCH plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses (2). In B cells, ITCH is thought to associate with latent membrane protein 2A (LMP2A) of Epstein-Barr virus, specifically down-regulating its activity in B cell signaling (3). Mutations in this gene are a cause of syndromic multisystem autoimmune disease (4).

Molekulargewicht: Predicted: 99 kDa

Observed: 99 kDa

Gen-ID: 83737

NCBI Accession: NP_001244066

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events