alpha Tubulin Antikörper (C-Term)

Produktnummer ABIN6991938

Dieses Anti-alpha Tubulin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von alpha Tubulin in WB, ELISA, IF und IHC (p). Geeignet für Human, Maus und Ratte.

Kurzübersicht für alpha Tubulin Antikörper (C-Term) (ABIN6991938)

Target: alpha Tubulin (TUBA1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser alpha Tubulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-alpha Tubulin Antikörper

Bindungsspezifität: C-Term

Spezifität: Alpha-Tubulin antibody is human, mouse and rat reactive.

Aufreinigung: alpha-Tubulin antibody is affinity chromatography purified via peptide column.

Immunogen: alpha-Tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-Tubulin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: alpha-Tubulin antibody can be used for detection of alpha-Tubulin by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human, mouse, and rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: alpha-Tubulin antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: alpha-Tubulin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu alpha Tubulin

Target: alpha Tubulin (TUBA1)

Andere Bezeichnung: alpha-Tubulin

Hintergrund: Alpha-Tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-Tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-Tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-Tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.

Molekulargewicht: Predicted: 50 kDa

Observed: 54 kDa

Gen-ID: 7846

Pathways: Microtubule Dynamics