alpha Tubulin Antikörper (C-Term)

Produktnummer ABIN6991938

Dieser Anti-alpha Tubulin Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von alpha Tubulin in WB, ELISA, IF und IHC (p). Geeignet für Human, Maus und Ratte.

Kurzübersicht für alpha Tubulin Antikörper (C-Term) (ABIN6991938)

Target: alpha Tubulin (TUBA1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser alpha Tubulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-alpha Tubulin Antikörper

Bindungsspezifität: C-Term

Spezifität: Alpha-Tubulin antibody is human, mouse and rat reactive.

Aufreinigung: alpha-Tubulin antibody is affinity chromatography purified via peptide column.

Immunogen: alpha-Tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-Tubulin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: alpha-Tubulin antibody can be used for detection of alpha-Tubulin by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human, mouse, and rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: alpha-Tubulin antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: alpha-Tubulin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu alpha Tubulin

Target: alpha Tubulin (TUBA1)

Andere Bezeichnung: alpha-Tubulin

Hintergrund: Alpha-Tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-Tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-Tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-Tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.

Molekulargewicht: Predicted: 50 kDa

Observed: 54 kDa

Gen-ID: 7846

Pathways: Microtubule Dynamics