ULK3 Antikörper (AA 230-280)

Produktnummer ABIN6991934

Der Kaninchen Polyklonal Anti-ULK3-Antikörper wurde für WB, ELISA, IHC (p) und IF validiert. Er ist geeignet, ULK3 in Proben von Human zu detektieren.

Kurzübersicht für ULK3 Antikörper (AA 230-280) (ABIN6991934)

Target: ULK3 (Unc-51-Like Kinase 3 (ULK3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ULK3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-ULK3 Antikörper

Bindungsspezifität: AA 230-280

Spezifität: ULK3 antibody is human specific. Multiple isoforms of ULK3 are known to exist.

Aufreinigung: ULK3 antibody is affinity chromatography purified via peptide column.

Immunogen: ULK3 antibody was raised against a 19 amino acid peptide near the center of human ULK3. The immunogen is located within amino acids 230 - 280 of ULK3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ULK3 antibody can be used for detection of ULK3 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: ULK3 antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: ULK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu ULK3

Target: ULK3 (Unc-51-Like Kinase 3 (ULK3))

Andere Bezeichnung: ULK3

Hintergrund: ULK3 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1). Knockout of ULK genes results in a severe defect in the autophagy pathway (2). ULK3, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK4, is highly conserved among eukaryotes (3). ULK3 has been shown to be a positive regulator of the Hedgehog signaling pathway by enhancing GLI1 and GLI2 transcriptional activity (4). Furthermore, ULK3 can also interact with SUFU, a protein required for the negative regulation of GLI proteins, this interaction blocks the autophosphorylation of ULK3 and blocks its ability to regulate the GLI proteins (5).

Molekulargewicht: Predicted: 52 kDa

Observed: 52 kDa

Gen-ID: 25989

NCBI Accession: NP_001092906

UniProt: Q6PHR2