Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
Spezifität
Multiple isoforms of KREMEN2 are known to exist. KREMEN2 antibody is predicted to not cross-react with other Kremen protein family members.
Aufreinigung
KREMEN2 Antibody is affinity chromatography purified via peptide column.
Immunogen
Anti-KREMEN2 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human KREMEN2. The immunogen is located within amino acids 390-440 of KREMEN2.
KREMEN2
Reaktivität: Maus, Ratte
WB, IF (cc), IF (p)
Wirt: Kaninchen
Polyclonal
Alexa Fluor 555
Applikationshinweise
WB: 1-2 μ,g/mL, ICC: 5 μ,g/mL.
Antibody validated: Western Blot in human, mouse and rat samples, Immunocytochemistry in human samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
KREMEN2 Antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
KREMEN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Target
KREMEN2
(Kringle Containing Transmembrane Protein 2 (KREMEN2))
KREMEN2 Antibody: Kremen (Kringle containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains and an intracellular region without any conserved motifs. Kremens bind a subset of the secreted Dickkopf proteins (Dkk 1, 2, and 4) with high affinity to modulate the canonical Wnt signaling pathway that is transduced by the ternary receptor complex composed of Wnt, Frizzled, and the LDL receptor related protein 5/6 (LRP5/6) co-receptor. Recent experiments have shown that KREMEN2 is a regulator of bone remodeling and raise the possibility that antagonizing KREMEN2 might prove beneficial in patients with bone loss disorders.