DPAGT1 Antikörper (N-Term)

Produktnummer ABIN6991580

Der Kaninchen Polyklonal anti-DPAGT1 Antikörper wird verwendet zum Nachweis von DPAGT1 in Proben von Human und Maus. Er wurde validiert für WB, ELISA, IHC (p) und IF.

Kurzübersicht für DPAGT1 Antikörper (N-Term) (ABIN6991580)

Target: DPAGT1 (Dolichyl-Phosphate (UDP-N-Acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P Transferase) (DPAGT1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DPAGT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-DPAGT1 Antikörper

Bindungsspezifität: AA 30-80, N-Term

Spezifität: At least four isoforms of DPAGT1 are known to exist, this antibody will recognize the two longest isoforms. DPAGT1 antibody is predicted to not cross-react with UHRF1BP1.

Aufreinigung: DPAGT1 Antibody is affinity chromatography purified via peptide column.

Immunogen: DPAGT1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human DPAGT1. The immunogen is located within amino acids 30 - 80 of DPAGT1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: DPAGT1 antibody can be used for detection of DPAGT1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: DPAGT1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: DPAGT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu DPAGT1

Target: DPAGT1 (Dolichyl-Phosphate (UDP-N-Acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P Transferase) (DPAGT1))

Andere Bezeichnung: DPAGT1

Hintergrund: DPAGT1 Antibody: The UDP-N-acetylglucosamine-dolichyl-phosphate N-acetyl-glucosaminephosphotransferase (DPAGT1) is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. Mutations in this integral endoplasmic reticulum (ER) membrane protein enzyme belongs to the glycosyltransferase family 4 results in the congenital disorder of glycosylation type Ij with symptoms such as severe hypotonia, medically intractable seizures, mental retardation, microcephaly, and exotropia. Recent experiments have shown that DPAGT1 is a target of the Wnt/beta-catenin signaling pathway, with Wnt3a inducing higher DPAGT1 mRNA expression.

Gen-ID: 1798

NCBI Accession: NP_001373

UniProt: Q9H3H5