ATG3 Antikörper (AA 180-230)

Produktnummer ABIN6991351

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch ATG3 in WB, ELISA, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für ATG3 Antikörper (AA 180-230) (ABIN6991351)

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ATG3 Antikörper

Bindungsspezifität: AA 180-230

Aufreinigung: ATG3 Antibody is affinity chromatography purified via peptide column.

Immunogen: ATG3 antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG3. The immunogen is located within amino acids 180 - 230 of ATG3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ATG3 antibody can be used for detection of ATG3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: ATG3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: ATG3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu ATG3

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Andere Bezeichnung: ATG3

Hintergrund: ATG3 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. It functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagy.

Gen-ID: 64422

NCBI Accession: NP_071933

UniProt: Q9NT62

Pathways: Autophagie