NOTUM Antikörper (N-Term)

Produktnummer ABIN6991166

Der Kaninchen Polyklonal Anti-NOTUM-Antikörper wurde für WB, ELISA, IHC (p) und IF validiert. Er ist geeignet, NOTUM in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für NOTUM Antikörper (N-Term) (ABIN6991166)

Target: NOTUM (Notum Pectinacetylesterase Homolog (NOTUM))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOTUM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-NOTUM Antikörper

Bindungsspezifität: AA 90-140, N-Term

Aufreinigung: NOTUM Antibody is affinity chromatography purified via peptide column.

Immunogen: NOTUM antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NOTUM. The immunogen is located within amino acids 90 - 140 of NOTUM.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: NOTUM antibody can be used for detection of NOTUM by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: NOTUM Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: NOTUM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu NOTUM

Target: NOTUM (Notum Pectinacetylesterase Homolog (NOTUM))

Andere Bezeichnung: NOTUM

Hintergrund: NOTUM Antibody: In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by beta-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.

Gen-ID: 147111

NCBI Accession: NP_848588

UniProt: Q6P988