NOTUM Antikörper (N-Term)

Produktnummer ABIN500375

Der Kaninchen Polyklonal anti-NOTUM Antikörper wird verwendet zum Nachweis von NOTUM in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für NOTUM Antikörper (N-Term) (ABIN500375)

Target: NOTUM (Notum Pectinacetylesterase Homolog (NOTUM))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOTUM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-NOTUM Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody detects Notum.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse, Rat.

Aufreinigung: Peptide Affinity Chromatography

Immunogen: NOTUM antibody was raised against a 19 amino acid peptide near the amino terminus of Human NOTUM.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu NOTUM

Target: NOTUM (Notum Pectinacetylesterase Homolog (NOTUM))

Andere Bezeichnung: Notum

Hintergrund: In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by b-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.

Gen-ID: 147111

NCBI Accession: NP_848588

UniProt: Q6P988