BORA Antikörper (N-Term)

Produktnummer ABIN6991094

Der Kaninchen Polyklonal Anti-BORA Antikörper wurde für WB, ELISA, IHC (p) und IF validiert. Er ist geeignet, BORA in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für BORA Antikörper (N-Term) (ABIN6991094)

Target: BORA (Protein Aurora Borealis (BORA))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BORA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-BORA Antikörper

Bindungsspezifität: AA 70-120, N-Term

Aufreinigung: Bora Antibody is affinity chromatography purified via peptide column.

Immunogen: Bora antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human Bora. The immunogen is located within amino acids 70 - 120 of Bora.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Bora antibody can be used for detection of Bora by Western blot at 1-2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Bora Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Bora antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu BORA

Target: BORA (Protein Aurora Borealis (BORA))

Andere Bezeichnung: Bora

Hintergrund: Bora Antibody: Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.

Gen-ID: 79866

UniProt: Q6PGQ7