BORA Antikörper (N-Term)

Produktnummer ABIN499491

Der Kaninchen Polyklonal anti-BORA Antikörper (ABIN499491) detektiert spezifisch BORA in WB, IHC (p) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Maus.

Kurzübersicht für BORA Antikörper (N-Term) (ABIN499491)

Target: BORA (Protein Aurora Borealis (BORA))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BORA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-BORA Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody reacts to BORA.

Aufreinigung: Affinity chromatography purified via peptide column

Immunogen: Bora antibody was raised against a 15 amino acid peptide from near the amino terminus of human Bora.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: Bora antibody can be used for detection of Bora at 1-2 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu BORA

Target: BORA (Protein Aurora Borealis (BORA))

Andere Bezeichnung: Protein Aurora Borealis

Hintergrund: Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.Synonyms: BORA, C13orf34, FLJ22624, HsBora

Gen-ID: 79866

UniProt: Q6PGQ7