AMBRA1 Antikörper (N-Term)

Produktnummer ABIN6990889

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch AMBRA1 in WB, ELISA, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für AMBRA1 Antikörper (N-Term) (ABIN6990889)

Target: AMBRA1 (Autophagy/beclin-1 Regulator 1 (AMBRA1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AMBRA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-AMBRA1 Antikörper

Bindungsspezifität: N-Term

Aufreinigung: Ambra1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Ambra1 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human Ambra1. The immunogen is located within the first 50 amino acids of Ambra1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Ambra1 antibody can be used for the detection of Ambra1 by Western blot at 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Ambra1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Ambra1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu AMBRA1

Target: AMBRA1 (Autophagy/beclin-1 Regulator 1 (AMBRA1))

Andere Bezeichnung: Ambra1

Hintergrund: Ambra1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.

Gen-ID: 55626

UniProt: Q9C0C7

Pathways: Autophagie