ATG12 Antikörper (N-Term)

Produktnummer ABIN6990837

Dieses Anti-ATG12-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ATG12 in WB, ELISA, IF und IHC (p). Geeignet für Human, Maus und Ratte.

Kurzübersicht für ATG12 Antikörper (N-Term) (ABIN6990837)

Target: ATG12 (Autophagy Related 12 (ATG12))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG12 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ATG12 Antikörper

Bindungsspezifität: N-Term

Aufreinigung: ATG12 Antibody is affinity chromatography purified via peptide column.

Immunogen: ATG12 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG12. The immunogen is located within the first 50 amino acids of ATG12.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ATG12 antibody can be used for the detection of ATG10 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: ATG12 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: ATG12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Andere Bezeichnung: ATG12

Hintergrund: ATG12 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5, this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.

Gen-ID: 9140

UniProt: O94817

Pathways: Autophagie