Endonuclease G Antikörper (N-Term)

Produktnummer ABIN6990798

Dieses Anti-Endonuclease G-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Endonuclease G in WB, ELISA, IHC (p) und IF. Geeignet für Human, Ratte und Maus.

Kurzübersicht für Endonuclease G Antikörper (N-Term) (ABIN6990798)

Target: Endonuclease G (ENDOG)

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Endonuclease G Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-Endonuclease G Antikörper

Bindungsspezifität: AA 40-90, N-Term

Aufreinigung: EndoG Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-EndoG antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human ENDOG. The immunogen is located within amino acids 40-90 of EndoG.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 0.5-2 μ,g/mL, IF: 20 μ,g/mL, IHC: 2.5-15 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: EndoG Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: EndoG antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu Endonuclease G

Target: Endonuclease G (ENDOG)

Andere Bezeichnung: EndoG

Hintergrund: EndoG Antibody: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndooG also cleaves DNA in vitro.

Molekulargewicht: Predicted: 33kD

Observed: 33kD kDa

Gen-ID: 2021

NCBI Accession: NP_004426

UniProt: Q14249

Pathways: Apoptose