TIGAR Antikörper (AA 60-110)

Produktnummer ABIN6990719

Der Kaninchen Polyklonal Anti-TIGAR-Antikörper wurde für ELISA, WB und IHC (p) validiert. Er ist geeignet, TIGAR in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für TIGAR Antikörper (AA 60-110) (ABIN6990719)

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TIGAR Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-TIGAR Antikörper

Bindungsspezifität: AA 60-110

Aufreinigung: TIGAR Antibody is affinity chromatography purified via peptide column.

Immunogen: TIGAR antibody was raised against a 18 amino acid synthetic peptide from near the center of human TIGAR. The immunogen is located within amino acids 60 - 110 of TIGAR.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: TIGAR antibody can be used for detection of TIGAR by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: TIGAR Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: TIGAR antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu TIGAR

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Andere Bezeichnung: TIGAR

Hintergrund: TIGAR Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death, loss of its functions contributes to the development of most cancers. Recent studies have demonstrated the ability of p53 to regulate the expression of several proteins involved in glycolysis and oxidative phosphorylation, such as TIGAR, SCO2, and phosphoglycerate mutase. TIGAR is a recently discovered protein that functions to regulate glycolysis and protect cells against oxidative stress. TIGAR is similar in structure to proteins in the phosphoglycerate mutase family, most notably 6-phosphofructo-2-kinase, suggesting TIGAR may function as a fructose bisphosphatase. Expression of TIGAR in transfected cells correlated with an inhibition of glycolysis and decreased levels of reactive oxygen species and p53-induced apoptosis, indicating that TIGAR may act to modulate the apoptotic response to p53, thereby allowing cells to survive mild or transient stresses.

Gen-ID: 57103

NCBI Accession: NP_065108

UniProt: Q9NQ88

Pathways: Warburg Effekt