IFIH1 Antikörper (C-Term)

Produktnummer ABIN6990714

Der Kaninchen Polyklonal anti-IFIH1 Antikörper (ABIN6990714) detektiert spezifisch IFIH1 in WB, ELISA, IF und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für IFIH1 Antikörper (C-Term) (ABIN6990714)

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IFIH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-IFIH1 Antikörper

Bindungsspezifität: C-Term

Aufreinigung: MDA5 Antibody is affinity chromatography purified via peptide column.

Immunogen: MDA5 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MDA5. The immunogen is located within the last 50 amino acids of MDA5.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: MDA5 antibody can be used for detection of MDA5 by Western blot at 1 - 4 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: MDA5 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: MDA5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu IFIH1

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Andere Bezeichnung: MDA5

Hintergrund: MDA5 Antibody: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-κ,B. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.

Molekulargewicht: Predicted: 113 kDa

Observed: 115 kDa

Gen-ID: 64135

NCBI Accession: NP_071451

UniProt: Q9BYX4

Pathways: Activation of Innate immune Response