LIPE Antikörper (C-Term)

Produktnummer ABIN6990685

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch LIPE in WB, ELISA, IHC (p) und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für LIPE Antikörper (C-Term) (ABIN6990685)

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIPE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-LIPE Antikörper

Bindungsspezifität: AA 920-970, C-Term

Spezifität: Multiple isoforms of Lipe are known to exist.

Aufreinigung: Lipe Antibody is affinity chromatography purified via peptide column.

Immunogen: Lipe antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Lipe. The immunogen is located within amino acids 920 - 970 of Lipe.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Lipe antibody can be used for detection of Lipe by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Lipe Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Lipe antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu LIPE

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Andere Bezeichnung: Lipe

Hintergrund: Lipe Antibody: Although initially described as an adipocyte-specific triacylglycerol lipase, Lipe (also known as hormone-specific lipase or HSL) is expressed in multiple tissues and cell lines. It plays multiple roles in lipid metabolism, including hormone-stimulated lipolysis in adipose tissue and the hydrolysis of cholesterol esters. Lipe is expressed as a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesterol esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. Recently, it was observed that the lack of Lipe in genetically obese leptin-null mice inhibited obesity and adipogenesis, suggesting that Lipe plays a major role in adipocyte proliferation.

Molekulargewicht: Predicted: 85, 90, 110, 118 kDa

Observed: 90 kDa

Gen-ID: 3991

UniProt: Q05469

Pathways: AMPK Signaling, Monocarboxylic Acid Catabolic Process, Lipid Metabolism