GAPDH Antikörper (N-Term)

Produktnummer ABIN6990628

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GAPDH in WB, ELISA, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für GAPDH Antikörper (N-Term) (ABIN6990628)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAPDH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GAPDH Antikörper

Bindungsspezifität: AA 60-110, N-Term

Aufreinigung: GAPDH Antibody is affinity chromatography purified via peptide column.

Immunogen: GAPDH antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human GAPDH. The immunogen is located within amino acids 60 - 110 of GAPDH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: GAPDH antibody can be used for the detection of GAPDH by Western blot at 0. 125 - 0.5 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: GAPDH Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung: GAPDH

Hintergrund: GAPDH Antibody: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Gen-ID: 2597

UniProt: P04406