AES Antikörper (C-Term)

Produktnummer ABIN6990571

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch AES in WB, ELISA, IF und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für AES Antikörper (C-Term) (ABIN6990571)

Target: AES (Amino-terminal Enhancer of Split (AES))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AES Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-AES Antikörper

Bindungsspezifität: C-Term

Aufreinigung: AES Antibody is affinity chromatography purified via peptide column.

Immunogen: AES antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human AES. The immunogen is located within the last 50 amino acids of AES.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: AES antibody can be used for the detection of AES by Western blot at 1 - 4 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: AES Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: AES antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu AES

Target: AES (Amino-terminal Enhancer of Split (AES))

Andere Bezeichnung: AES

Hintergrund: AES Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

Gen-ID: 166

NCBI Accession: NP_945320

UniProt: Q08117

Pathways: WNT Signalweg, SARS-CoV-2 Protein Interaktom