RHEB Antikörper (Middle Region)

Produktnummer ABIN6990534

Dieses Anti-RHEB-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von RHEB in WB, ELISA, IF und IHC (p). Geeignet für Human, Maus und Ratte.

Kurzübersicht für RHEB Antikörper (Middle Region) (ABIN6990534)

Target: RHEB (Ras Homolog Enriched in Brain (RHEB))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RHEB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-RHEB Antikörper

Bindungsspezifität: AA 40-90, Middle Region

Aufreinigung: Rheb Antibody is affinity chromatography purified via peptide column.

Immunogen: Rheb antibody was raised against a 15 amino acid synthetic peptide from the middle region of human Rheb. The immunogen is located within amino acids 40 - 90 of Rheb.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Rheb antibody can be used for the detection of Rheb by Western blot at 1 to 4 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rheb Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Rheb antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu RHEB

Target: RHEB (Ras Homolog Enriched in Brain (RHEB))

Andere Bezeichnung: Rheb

Hintergrund: Rheb Antibody: Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.

Molekulargewicht: 19, 20 kDa

Gen-ID: 6009

UniProt: Q15382

Pathways: RTK Signalweg