PUMA Antikörper (Isoform 1, N-Term)

Produktnummer ABIN6990344

Der Kaninchen Polyklonal anti-PUMA Antikörper wird verwendet zum Nachweis von PUMA in Proben von Human und Ratte. Er wurde validiert für WB, ELISA, IF und IHC (p).

Kurzübersicht für PUMA Antikörper (Isoform 1, N-Term) (ABIN6990344)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PUMA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PUMA Antikörper

Bindungsspezifität: Isoform 1, N-Term

Aufreinigung: PUMA Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-PUMA antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human PUMA isoform 1. The immunogen is located within the first 50 amino acids of PUMA.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 2-3 μ,g/mL, IF: 10-20 μ,g/mL, IHC: 2.5-10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PUMA Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: PUMA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Andere Bezeichnung: PUMA

Hintergrund: PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53 (1). The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (2). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Molekulargewicht: Predicted: 21kD

Observed: 23kD kDa

Gen-ID: 27113

NCBI Accession: NP_055232

UniProt: Q96PG8

Pathways: p53 Signalweg, Positive Regulation of Endopeptidase Activity